DFFA Polyclonal Antibody

DFFA Polyclonal Antibody SKU # E-AB-10383 Reactivity Human Host Rabbit Applications IHC Product Details Isotype IgG Host Rabbit Reactivity Human Applications IHC Clonality Polyclonal Immunogen Recombinant protein of human DFFA Abbre DFFA Synonyms A330085O09Rik,  Caspase activated deoxyribonuclease inhibitor short form,  DFF 1,  DFF 45,  DFF alpha,  DFF-45,  DFF1,  DFF35,  DFF45,  DFFA,  DNA ,  DNA Fragmentation Factor Alpha Subunit,  DNA fragmentation factor 45 kDa subunit,  Dffa DNA fragmentation factor,  alpha subunit Swissprot O00273 Cellular Localization Cytoplasm. Concentration 0.7 mg/mL Buffer Phosphate buffered solution, pH 7.4, containing 0.05% stabilizer and 50% glycerol. Purification Method Affinity purification Research Areas Cancer,  Cell Biology,  Epigenetics and Nuclear Signaling,  Metabolism Conjugation Unconjugated Storage Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles. Shipping The product is shipped with ice pack,upon receipt,store it immediately at the temperature recommended. Related Reagents Applications Recommended Dilution IHC 1:30-1:150 Background Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.