2-Step Plus Poly-HRP Anti Rabbit/Mouse IgG Detection System (with DAB Solution）

2-Step Plus Poly-HRP Anti Rabbit/Mouse IgG Detection System (with DAB Solution） SKU # E-IR-R213 Applications IHC Product Details Product Introduction 2-step plus is a two-step immunohistochemical broad spectrum detection reagent. It polymerizes monovalent Fab fragments of secondary antibody and enzyme, which replaces the secondary antibody and tertiary antibody in traditional method, can directly amplify the binding signal of antibody-antigen. This method not only retains the specific binding ability of antibody with antigen, but also can effectively avoid space steric hindrance caused by excessive polymer molecules. Compared with the traditional SP three-step method, this kit has the characteristics of simple, rapid, high-sensitivity. This system abandons the using of biotin, so it can avoid background staining by endogenous biotin. It can be used in IHC, in which the primary antibody is monoclonal/polyclonal antibody derived from rabbit/mouse. The unique polymer auxiliary agent for macromolecular detection system can detect the binding primary antibody better.Diluent for DAB concentrated solution has been provided in this kit to avoid the influence of the different water acidity and alkalinity on the DAB Chromogenic Agent. Applications IHC Storage Store at 2~8°C, shading light. Avoid of freezing. Valid for 12 months. The reagents are valid within 6 months after opening. Shipping Biological ice pack at 4 ℃ Notes 1. This product couldn’t be used for clinical diagnosis or treatment, food or medicine, and can’t be stored in residence.2. For your safety and health, please wear the lab coat and disposable gloves before the experiments.